We seek to identify novel genes and pathways in the pathogenesis of nanophthalmos, a condition characterized by small, but structurally normal eyes. We have recently identified the membrane associated transcription factor myelin regulatory factor (MYRF) as a novel gene in the pathogenesis of this condition. In a large cohort of nanophthalmos and hyperopia patients, we identified genetic diagnoses in less than 20% of families, leaving 80% of cases currently genetically unexplained despite strong heritability of this condition. We work with cohorts in the US and have an collaborative effort in Brazil with Pedro Carricondo, Marianna Hollaender, and Fernanda Abalem. Ongoing efforts include the following:
Identifying downstream targets of MYRF and screening them as possible culprits for nanophthalmos and other inherited retinal disease using cutting edge genomic techniques including scRNA sequencing and CUT&RUN sequencing.
Identifying novel candidate genes for nanophthalmos, inherited retinal disease, and other eye size related phenotypes such as angle closure glaucoma using panel-based, whole exome, and whole genome sequencing from large cohorts of collected patients and families.
Our work with a conditional knockout mouse model of MYRF revealed a role for MYRF in RPE development. Mice with loss of Myrf in the early eye field (using Rx>Cre transgene excision of a floxed Myrf allele) display pigmentation defects in the RPE, loss of photoreceptor outer segment formation, and subsequent retinal degeneration. Ongoing efforts are aimed at understanding the role of MYRF in RPE development and maintenance at the cellular and molecular level using scRNA sequencing and a combination of histologic, biochemical, and cell biology approaches. We are collaborating also with Jason Miller's laboratory to define the role of MYRF, its downstream targets, and disease associated variants, in human RPE culture models.
We are using high-throughput sequencing approaches to identify novel candidate genes for pediatric glaucoma, including primary congenital (PCG) and juvenile open angle glaucoma (JOAG). We use a combination of traditional linkage and linkage exclusion analysis along with whole exome and whole genome sequencing to define candidate genes. These are subsequently be validated using cell culture and animal models. A sample pedigree with JOAG is shown along with whole genome linkage exclusion data.
We recently identified a novel RIGI/DDX58 variant in several families with Singleton-Merten syndrome, a rare disorder featuring juvenile open angle glaucoma, skeletal defects, aortic calcifications, and a psoriasiform rash. RIGI is an RNA-binding protein that serves as a pattern recognition receptor, binding to viral RNA and activating type I interferon signaling as part of the innate immune system. Variants associated with Singleton-Merten syndrome lead to aberrant activation of this pathway in specific tissues, and there is significant variability. Ongoing efforts are aimed at identifying the molecular mechanism of glaucoma, identifying the mechanisms that lead to differential tissue involvement and variable penetrance, and identifying optimal treatments for this disorder (by targeting the downstream Type I interferon signaling pathway.
Together with Shahzad Mian and Yan Zhang's research group, we are developing adenine base editing therapies for TGFBI corneal dystrophy, optimizing corneal delivery for these therapies, and defining the patients that would benefit from this therapy.
Dr. Prasov working together with Pediatric Medical Genetics leads a clinic that specializes in the diagnosis and management of inherited ocular disorders. We lead and contribute to studies involving new genetic associations, novel disease phenotypes, and genotype-phenotype correlation or natural history.
We are always looking for students and postdoctoral fellows from a variety of backgrounds to join the lab. Dr. Prasov is a member of the Genetics and Genomics (G&G) and Cell and Molecular Biology (CMB) Programs as part of the The Program in Biomedical Sciences (PIBS) Ph.D. graduate training program at the University of Michigan. We are looking to recruit motivated and energetic graduate, medical, medical scientist training program (MSTP) students, and postdoctoral fellows. Graduate and MD/PhD students, and ophthalmology residents and fellows interested in join us should email to set up a time to talk. Post-doctoral candidates should include 3 letters of reference and a brief, 1 page statement summarizing their research interests and goals. If you're interested in joining our team, please email and send us your CV.